Collagen IV anticorps
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- Antigène Voir toutes Collagen IV (COL4) Anticorps
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Collagen IV est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)
- Specificité
- Human collagen type IV 100 % human collagen types I, II, III, and V <0.1 % human fibronectin and laminin < 0.1 % No cross-reactivity with bovine
- Aucune reactivité croisée
- Boeuf (Vache), Souris, Porc, Rat
- Réactivité croisée (Details)
- Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV
- Attributs du produit
- Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100 % , human collagen types I, II, III, and V <0.1 %, human fibronectin and laminin < 0.1 % (RIA at 1:10000 dilution).
- Purification
- Protein G affinity purified antibody prod. in nude mice.
- Immunogène
- Purified collagen type IV from human placenta
- Clone
- 24D1
- Isotype
- IgG1
- Top Product
- Discover our top product COL4 Anticorps primaire
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- Indications d'application
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:320, IHC(P) ? 1:4000, ELISA ? 1:4000 (OD ? 500)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- phosphate buffered solution, no BSA and preservative added!
- Agent conservateur
- Without preservative
- Stock
- -20 °C
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- Antigène
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Autre désignation
- Collagen Type IV (COL4 Produits)
- Synonymes
- anticorps collagen, type IV, anticorps Let-2
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