Histone 3 anticorps (H3K4me, pThr6)

N° du produit ABIN5706876

Détail du produit anti-Histone 3 anticorps

Antigène: Histone 3 (H3)

Épitope: H3K4me, pThr6

Reactivité: Humain, Souris, Rat, Poulet, Xenopus laevis, C. elegans, Drosophila melanogaster, Plantes

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Attributs du produit:

Synonyms: rabbit anti-Histone H3 monomethyl Lys4 pT6 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me1/pT6

Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, trimethylation of lysine 4 on H3 (H3 K4Me3) is a well known mark of gene activation. However, the role of phosphorylation at threonine 6 on H3 (H3 pT6) is more obscure. Yet recently, the two modifications have been shown to interact with each other. When H3 T6 is phosphorylated by protein kinase C beta 1 (PRKCbeta), the histone demethylase LSD1 is prevented from removing methyl groups from H3 K4. This same study also correlated high levels of pT6 and PRKCbeta as a possible marker for prostate cancer, as well as tumor progression in xenografts. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene Name: HIST2H3C

Purification: Anti-Histone H3 [Monomethyl Lys4, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Immunogène:

Immunogen: Histone H3 [Monomethyl Lys4, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic monomethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H3 [Monomethyl Lys4, p Thr6] antibody is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.55 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 30 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Références pour anticorps anti-Histone 3 (ABIN5706876)

Mika, Luelling, Pavek, Nartker, Heyneman, Jones, Barrott: "Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma." dans: Journal of clinical medicine, Vol. 8, Issue 3, (2019) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3)

Autre désignation: Histone H3 (H3 Produits)

Synonymes: anticorps H-3, anticorps histocompatibility 3, anticorps H3

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3