APOB anticorps

N° du produit ABIN614989

Détail du produit anti-APOB anticorps

Antigène: APOB (Apolipoprotein B (APOB))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APOB est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: Typically less than 1 % cross reactivity againstother types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Immunoaffinity Chromatography

Immunogène: ApoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification, Genename: APOB

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2 - 8 °C prior to opening. Following opening store (in aliquots) at -20 °C. For extended storage mix with an equal volume of glycerol. Dilute only prior to immediate use.

Détails sur APOB

Antigène: APOB (Apolipoprotein B (APOB))

Autre désignation: Apolipoprotein B (Apo B) (APOB Produits)

Synonymes: anticorps FLDB, anticorps LDLCQ4, anticorps Ac1-060, anticorps Apo B-100, anticorps ApoB-100, anticorps ApoB-48, anticorps AI315052, anticorps apob-100, anticorps apob-48, anticorps APOB-100, anticorps ApoB(100), anticorps APOB, anticorps apolipoprotein B, anticorps APOB, anticorps Apob

Sujet: Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence. In the ApoB-100 isoform the precursor has 4563 amino acids, and the mature apoB-100 has 4536 amino acid residues. Mature, circulating B-48 is homologous over its entire length (estimated to be between 2130 and 2144 amino acid residues) with the amino-terminal portion of B-100 and contains no sequence from the carboxyl end of B-100. From structural studies, it is thought that apoB-48 represents the amino-terminal 47 % of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of apoB-100. Apolipoprotein B-48 may be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C-to-U change in the codon CAA encoding Gln(2153) in apoB-100 mRNA. Since only the sequence that codes B-100 is present in genomic DNA, this presents the possibility of an organ-specific introduction of a stop codon to an mRNA and the change from CAA to UAA of codon 2153 of the message as a unique RNA editing process.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100

ID gène: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism