Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) anticorps (CF®568)
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- Antigène Voir toutes Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) Anticorps
- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
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Épitope
- AA 356-475
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- CF®568
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Fonction
- Mouse Monoclonal anti-Spectrin-beta-III (SPTBN2/1583), CF568 Conjugate
- Attributs du produit
- Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin ? and spectrin ? are present in erythrocytes, whereas spectrin ? II (also designated fodrin ?) and spectrin ? I (also designated fodrin ?) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ? III is highly homologous to both spectrin ? I and spectrin ? II. Spectrin ? III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)
- Clone
- SPTBN2-1583
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product SPTBN2 Anticorps primaire
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- Indications d'application
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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HeLa cells. Pancreas or Liver.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
- Autre désignation
- Spectrin-beta-III (SPTBN2 Produits)
- Synonymes
- anticorps Spnb3, anticorps mKIAA0302, anticorps GTRAP41, anticorps SCA5, anticorps spectrin beta, non-erythrocytic 2, anticorps spectrin, beta, non-erythrocytic 2, anticorps Sptbn2, anticorps SPTBN2
- Sujet
- Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2
- Poids moléculaire
- 246 kDa
- ID gène
- 6712, 26915
- UniProt
- O15020
- Pathways
- Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
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