CDC20 anticorps
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- Antigène Voir toutes CDC20 Anticorps
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CDC20 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-Cdc20 (CDC20/1102), Purified, BSA-free
- Attributs du produit
- Cyclins, regulatory subunits that associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogène
- Recombinant human Cdc20 protein
- Clone
- CDC20-1102
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDC20 Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 2-4 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Ramos or HeLa cells. Tonsil or gastric carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Agent conservateur
- Azide free
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- Antigène
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- Autre désignation
- Cdc20 (CDC20 Produits)
- Synonymes
- anticorps X-FZY, anticorps ba276h19.3, anticorps cdc20a, anticorps fizzy1, anticorps p55cdc, anticorps CDC20A, anticorps bA276H19.3, anticorps p55CDC, anticorps 2310042N09Rik, anticorps C87100, anticorps cell division cycle 20, anticorps cell division cycle 20 L homeolog, anticorps cell division cycle 20 homolog (S. cerevisiae), anticorps cdc20, anticorps CDC20, anticorps cdc20.L, anticorps Cdc20
- Sujet
- CDC20, CDC20A, p55CDC, P55CDC-LSB
- Poids moléculaire
- 55 kDa
- ID gène
- 991, 524947
- UniProt
- Q12834
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