Neurofilament anticorps (CF®640R)
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- Antigène Tous les produits Neurofilament
- Neurofilament
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Reactivité
- Humain, Rat, Souris, Poulet, Porc
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Neurofilament est conjugé à/à la CF®640R
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-Neurofilament (H+L) (RT-97 + NR-4), CF640R Conjugate
- Attributs du produit
- This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Triton-X 100 insoluble proteins of rat brain (RT-97), Neurofilaments from porcine spinal cord (NR-4)
- Clone
- RT-97 NR-4
- Isotype
- IgG
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- Indications d'application
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Cerebellum, Neuroblastoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- Neurofilament
- Abstract
- Neurofilament Produits
- Poids moléculaire
- 200 kDa & 68 kDa
- ID gène
- 4744, 198760
- UniProt
- P12036
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