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Neurofilament anticorps (CF®640R)

Reactivité: Humain, Rat, Souris, Poulet, Porc FACS, IHC (f) Hôte: Souris Monoclonal RT-97 NR-4 CF®640R
N° du produit ABIN6188780
  • Antigène Tous les produits Neurofilament
    Neurofilament
    Reactivité
    • 27
    • 24
    • 21
    • 15
    • 15
    • 3
    Humain, Rat, Souris, Poulet, Porc
    Hôte
    • 22
    • 7
    • 1
    Souris
    Clonalité
    • 22
    • 8
    Monoclonal
    Conjugué
    • 16
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Neurofilament est conjugé à/à la CF®640R
    Application
    • 18
    • 14
    • 9
    • 7
    • 7
    • 5
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Fonction
    Mouse Monoclonal anti-Neurofilament (H+L) (RT-97 + NR-4), CF640R Conjugate
    Attributs du produit
    This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogène
    Triton-X 100 insoluble proteins of rat brain (RT-97), Neurofilaments from porcine spinal cord (NR-4)
    Clone
    RT-97 NR-4
    Isotype
    IgG
  • Indications d'application
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Commentaires

    Cerebellum, Neuroblastoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Protect from light
  • Antigène
    Neurofilament
    Abstract
    Neurofilament Produits
    Poids moléculaire
    200 kDa & 68 kDa
    ID gène
    4744, 198760
    UniProt
    P12036
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