CD63 anticorps

N° du produit ABIN6252768

Détail du produit anti-CD63 anticorps

Antigène: CD63

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purification: Protein G affinity chromatography

Immunogène: Full length human CD63 was used as the immunogen for this antibody.

Clone: MX-49-129-5

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

WB: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD63

Antigène: CD63

Autre désignation: CD63 (CD63 Produits)

Synonymes: anticorps C75951, anticorps ME491, anticorps Tspan30, anticorps LAMP-3, anticorps MLA1, anticorps OMA81H, anticorps TSPAN30, anticorps TSP29FA, anticorps CD63 antigen, anticorps CD63 molecule, anticorps Cd63 molecule, anticorps Cd63, anticorps CD63

Sujet: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

ID gène: 967

UniProt: P08962