VCP anticorps (Internal Region)

N° du produit ABIN6266291

Détail du produit anti-VCP anticorps

Antigène: VCP (Valosin Containing Protein (VCP))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VCP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: VCP Antibody detects endogenous levels of total VCP.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human VCP, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:100, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur VCP

Antigène: VCP (Valosin Containing Protein (VCP))

Autre désignation: VCP (VCP Produits)

Synonymes: anticorps ALS14, anticorps IBMPFD, anticorps TERA, anticorps p97, anticorps CDC48, anticorps wu:fd16d05, anticorps wu:fj63d11, anticorps p93, anticorps 3110001E05, anticorps p97/VCP, anticorps 22.26, anticorps 7.12, anticorps Apple, anticorps BcDNA.GM02885, anticorps BcDNA:GM02885, anticorps CG2331, anticorps DmTER94, anticorps Dmel\CG2331, anticorps I, anticorps Ter94, anticorps Tera, anticorps VCP, anticorps VCP ATPase, anticorps VCP/p97, anticorps Vcp, anticorps anon-WO2004063362.65, anticorps anon-WO2004063362.67, anticorps cdc48, anticorps dTERA, anticorps dVCP, anticorps dmTERA, anticorps dvcp, anticorps l(2)03775, anticorps l(2)46CFf, anticorps l(2)46CFs, anticorps l(2)46Ch, anticorps pTER94, anticorps ter94, anticorps vcp, anticorps FANCG, anticorps valosin containing protein, anticorps valosin containing protein S homeolog, anticorps valosin-containing protein, anticorps CG2331 gene product from transcript CG2331-RA, anticorps zgc:136908, anticorps transitional endoplasmic reticulum ATPase, anticorps VCP, anticorps vcp.S, anticorps vcp, anticorps Vcp, anticorps Bm1_56585, anticorps LOC100136246, anticorps TER94, anticorps zgc:136908, anticorps LOC101109680

Sujet:

Description: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation (PubMed:26565908). Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites (PubMed:22020440, PubMed:22120668). Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage (PubMed:23042607, PubMed:23042605). Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation (PubMed:16186510, PubMed:21118995). Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy (PubMed:20104022, PubMed:27753622). Acts as a negative regulator of type I interferon production by interacting with DDX58/RIG-I: interaction takes place when DDX58/RIG-I is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58/RIG-I (PubMed:26471729). May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation (PubMed:21822278). May more particularly play a role in caveolins sorting in cells (PubMed:21822278, PubMed:23335559).

Gene: VCP

Poids moléculaire: 71kDa

ID gène: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway