BRCA2 anticorps (AA 21-130) (Biotin)

N° du produit ABIN673436

Détail du produit anti-BRCA2 anticorps (Biotin)

Antigène: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Épitope: AA 21-130

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRCA2 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human BRCA2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur BRCA2

Antigène: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Autre désignation: BRCA2 (BRCA2 Produits)

Synonymes: anticorps BRCC2, anticorps BROVCA2, anticorps FACD, anticorps FAD, anticorps FAD1, anticorps FANCB, anticorps FANCD, anticorps FANCD1, anticorps GLM3, anticorps PNCA2, anticorps 30169, anticorps BcDNA:SD25109, anticorps CG13583, anticorps CG13584, anticorps CG30169, anticorps Dmel\\CG30169, anticorps brca2, anticorps dmbrca2, anticorps fancd1, anticorps BRCA2, anticorps brcc2, anticorps brovca2, anticorps facd, anticorps fad, anticorps fad1, anticorps fancb, anticorps AI256696, anticorps AW045498, anticorps Fancd1, anticorps RAB163, anticorps BRCA2, DNA repair associated, anticorps Breast cancer 2, early onset homolog, anticorps breast cancer 2, early onset, anticorps BRCA2, anticorps Brca2, anticorps brca2, anticorps LOC584780

Sujet:

Synonyms: FAD, FACD, FAD1, GLM3, BRCC2, FANCD, PNCA2, FANCD1, BROVCA2, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, BRCA2

Background: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).

ID gène: 675

UniProt: P51587

Pathways: Réparation de l'ADN, M Phase, Maintenance of Protein Location