TCF7L1 anticorps (AA 35-84)

N° du produit ABIN6741527

Détail du produit anti-TCF7L1 anticorps

Antigène: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Épitope: AA 35-84

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TCF7L1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human TCF7L1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Guinea pig (100%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%), Monkey, Elephant, Opossum (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur TCF7L1

Antigène: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Autre désignation: TCF7L1 (TCF7L1 Produits)

Synonymes: anticorps TCF-3, anticorps TCF3, anticorps Tcf-3, anticorps Tcf3, anticorps bHLHb21, anticorps tcf3c, anticorps tcf3d, anticorps xtcf3, anticorps XTCF-3, anticorps tcf7l1, anticorps tcf3b, anticorps TCF-3-A, anticorps tcf-3, anticorps tcf3, anticorps tcf3a, anticorps zTcf-3, anticorps transcription factor 7 like 1, anticorps transcription factor 7 like 1 (T cell specific, HMG box), anticorps transcription factor 7 like 1 L homeolog, anticorps transcription factor 7 like 1 S homeolog, anticorps transcription factor 7 like 1b, anticorps transcription factor 7 like 1a, anticorps TCF7L1, anticorps Tcf7l1, anticorps tcf7l1.L, anticorps tcf7l1.S, anticorps tcf7l1b, anticorps tcf7l1a

Sujet: Name/Gene ID: TCF7L1

Synonyms: TCF7L1, TCF-3, Transcription factor 7-like 1, HMG box transcription factor 3

ID gène: 83439

NCBI Accession: NP_112573

UniProt: Q9HCS4

Pathways: Signalisation WNT, Stem Cell Maintenance