TGFB1I1 anticorps (N-Term)

N° du produit ABIN6741575

Détail du produit anti-TGFB1I1 anticorps

Antigène: TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TGFB1I1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human TGFB1I1 / HIC-5

Homologie: Percent identity by BLAST analysis: Mouse, Rat, Dog, Rabbit (100%) Horse (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human TGFB1I1 (B2R8D5, NP_057011). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Panda, Dog, Rabbit (100%), Marmoset, Horse (92%), Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Tested in Western blot using transfected cells. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur TGFB1I1

Antigène: TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))

Autre désignation: ARA55 / HIC-5 (TGFB1I1 Produits)

Synonymes: anticorps TGFB1I1, anticorps hic5, anticorps ara55, anticorps hic-5, anticorps tsc-5, anticorps ARA55, anticorps HIC-5, anticorps HIC5, anticorps TSC-5, anticorps Hic5, anticorps Ara55, anticorps Hic-5, anticorps ara-55, anticorps transforming growth factor beta 1 induced transcript 1, anticorps transforming growth factor beta 1 induced transcript 1 L homeolog, anticorps TGFB1I1, anticorps tgfb1i1, anticorps Tgfb1i1, anticorps tgfb1i1.L

Sujet: Name/Gene ID: TGFB1I1
Family: TGF beta

Synonyms: TGFB1I1, ARA55, HIC5, HIC-5, TSC-5

ID gène: 7041

NCBI Accession: NP_057011

UniProt: O43294

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, VEGF Signaling