TRPM2 anticorps (AA 431-480)

N° du produit ABIN490062

Détail du produit anti-TRPM2 anticorps

Antigène: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Épitope: AA 431-480

Reactivité: Humain, Souris, Rat, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRPM2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human TRPM2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Horse (92%) Guinea pig (87%) Chicken (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa431-480 of human TRPM2 (O94759, NP_003298). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%), Galago, Marmoset, Elephant, Bovine, Bat, Horse (92%), Guinea pig (87%), Panda, Platypus, Lizard (85%), Turkey, Zebra finch, Chicken (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 - 20 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur TRPM2

Antigène: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Autre désignation: TRPM2 (TRPM2 Produits)

Synonymes: anticorps EREG1, anticorps KNP3, anticorps LTRPC2, anticorps NUDT9H, anticorps NUDT9L1, anticorps TRPC7, anticorps Trpm2-predicted, anticorps TRPM2, anticorps si:ch73-263b18.1, anticorps 9830168K16Rik, anticorps C79133, anticorps Trp7, anticorps Trrp7, anticorps transient receptor potential cation channel subfamily M member 2, anticorps transient receptor potential cation channel, subfamily M, member 2, anticorps transient receptor potential cation channel subfamily C member 7, anticorps TRPM2, anticorps Trpm2, anticorps trpm2, anticorps TRPC7

Sujet: Name/Gene ID: TRPM2
Subfamily: Calcium channel - TRP
Family: Ion Channel

Synonyms: TRPM2, EREG1, LTrpC-2, LTRPC2, NUDT9L1, KNP3, NUDT9H, Trrp7

ID gène: 7226

NCBI Accession: NP_003298

UniProt: O94759