CD4 anticorps (violetFluor™ 500)
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- Antigène Voir toutes CD4 Anticorps
- CD4
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD4 est conjugé à/à la violetFluor™ 500
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Application
- Flow Cytometry (FACS)
- Réactivité croisée
- Chimpanzé
- Purification
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- Clone
- RPA-T4
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD4 Anticorps primaire
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- Indications d'application
- This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500, eFluor® 506 or Pacific Orange®.
- Commentaires
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5 uL (0.5 ug)/test
- Restrictions
- For Research Use only
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- 2-8°C protected from light
- Date de péremption
- 12 months
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- Antigène
- CD4
- Autre désignation
- CD4 (CD4 Produits)
- Synonymes
- anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
- Sujet
- The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).
- ID gène
- 920
- UniProt
- P06332
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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