NF-kB p65 anticorps (pSer529)

N° du produit ABIN6972429

L’anticorps Lapin Polyclonal anti-NF-kB p65 a été validé pour WB. Il convient pour détecter NF-kB p65 dans des échantillons de Humain.

Aperçu rapide pour NF-kB p65 anticorps (pSer529) (ABIN6972429)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NF-kB p65 anticorps

Épitope: pSer529

Attributs du produit: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA. NFkB p65 phospho Ser529 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogène: This NFkB p65 phospho Ser529 antibody was raised against a peptide containing phospho-serine 529 of human NFkB p65.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NFκB p65

Poids moléculaire: 65 kDa

NCBI Accession: NP_068810

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins