Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
CCDC98 Antibody is affinity chromatography purified via peptide column.
Immunogène
CCDC98 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human CCDC98. The immunogen is located within amino acids 80 - 130 of CCDC98.
FAM175A
Reactivité: Souris
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Cy5
Indications d'application
CCDC98 antibody can be used for detection of CCDC98 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
CCDC98 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
CCDC98 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
CCDC98 (FAM175A)
(Family with Sequence Similarity 175, Member A (FAM175A))
anticorps CCDC98, anticorps abra1, anticorps ccdc98, anticorps fam175a, anticorps ABRA1, anticorps 3830405G04Rik, anticorps 5630400M01Rik, anticorps AI506069, anticorps AL024423, anticorps AV118690, anticorps Ccdc98, anticorps RGD1305287, anticorps zgc:103522, anticorps abraxas 1, BRCA1 A complex subunit, anticorps Coiled-coil domain-containing protein 98, anticorps BRCA1 A complex subunit, anticorps family with sequence similarity 175 member A, anticorps abraxas 2a, BRISC complex subunit, anticorps abraxas 1, BRCA1 A complex subunit S homeolog, anticorps ABRAXAS1, anticorps abraxas1, anticorps Abraxas1, anticorps FAM175A, anticorps abraxas2a, anticorps abraxas1.S
Sujet
CCDC98 Antibody: CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.