PKC alpha anticorps (C-Term)

N° du produit ABIN7126832

Cet anticorps Souris Monoclonal détecte spécifiquement PKC alpha dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PKC alpha anticorps (C-Term) (ABIN7126832)

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 133

Détail du produit anti-PKC alpha anticorps

Épitope: C-Term

Fonction: Protein Kinase C α / PRKCA Antibody

Specificité: Protein Kinase C alpha (PKC°™) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the °™ isoform of PKC°™ and binds to a sequence at the C terminus of PKC°™ to detect its expression in vitro.

Réactivité croisée (Details): Human, Rat and Mouse.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCα.

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Normal human kidney or tonsil tissue. HEK293 whole cell lysates.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur PKC alpha

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Autre désignation: PRKCA

Sujet:

PKC alpha, PKC-A, PKC-alpha, PKCA, PRKCA, Protein kinase C alpha, Protein kinase C alpha type

Normal human kidney or tonsil tissue. HEK293 whole cell lysates.

Poids moléculaire: 77kDa

ID gène: 5578, 531704, 708867

UniProt: P17252

Pathways: Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling