H2AFX anticorps (pSer139)

N° du produit ABIN7138424

Détail du produit anti-H2AFX anticorps

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Épitope: pSer139

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Réactivité croisée: Humain

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogène: Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:1000, IF:1:100-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: H2AFX (H2AFX Produits)

Synonymes: anticorps H2A.X, anticorps H2A/X, anticorps H2AX, anticorps AW228881, anticorps H2ax, anticorps Hist5-2ax, anticorps gammaH2ax, anticorps zgc:56329, anticorps h2a.x, anticorps h2a/x, anticorps h2ax, anticorps RGD1566119, anticorps h2a, anticorps h2afx, anticorps H2A histone family member X, anticorps H2A histone family, member X, anticorps H2A histone family member X L homeolog, anticorps histone cluster 1, H2ah, anticorps histone cluster 2, H2ab S homeolog, anticorps histone H2AX, anticorps H2AFX, anticorps H2afx, anticorps h2afx, anticorps h2afx.L, anticorps HIST1H2AH, anticorps hist2h2ab.S, anticorps LOC100522201, anticorps LOC100720536

Sujet:

Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript, available in PMC 2006 March 6.

Aliases: H2A histone family member X antibody, H2A histone family member X antibody, H2A.FX antibody, H2A.X antibody, H2a/x antibody, H2AFX antibody, H2AX antibody, H2AX_HUMAN antibody, Histone H2A.X antibody

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus