Recombinant CD25 anticorps
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- Antigène Voir toutes CD25 (IL2RA) Anticorps
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD25 est non-conjugé
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), In vivo Studies (in vivo)
- Fonction
- In vivo Grade Recombinant Anti-mouse CD25 rat IgG1 Lamda Monoclonal Antibody
- Attributs du produit
- The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.
- Purification
- Protein G affinity column
- Pureté
- > 95% by SDS-PAGE under reducing conditions and HPLC.
- Stérilité
- 0.2 μm filtered
- niveau d'endotoxine
- < 1 EU per 1 mg of the protein by the LAL method.
- Classe de qualité
- In vivo Grade
- Isotype
- IgG1 lamda
- Top Product
- Discover our top product IL2RA Anticorps primaire
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- Indications d'application
- immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4
- Conseil sur la manipulation
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Stock
- -20 °C
- Stockage commentaire
- 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
- Date de péremption
- 3 months
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- Antigène
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Autre désignation
- CD25 (IL2RA Produits)
- Synonymes
- anticorps CD25, anticorps IDDM10, anticorps IL2R, anticorps TCGFR, anticorps IL2RAC, anticorps IL-2RA, anticorps Il2r, anticorps Ly-43, anticorps interleukin 2 receptor subunit alpha, anticorps interleukin-2 receptor subunit alpha-like, anticorps interleukin 2 receptor, alpha chain, anticorps IL2RA, anticorps Il2ra, anticorps LOC100222957
- Pathways
- Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation
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