NF-kB p65 anticorps (pSer536)

N° du produit ABIN730528

Cet anticorps Lapin Polyclonal détecte spécifiquement NF-kB p65 dans WB, ELISA, IHC (p) et FACS. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache) et Poulet et a été mentionné dans 12+ publications.

Aperçu rapide pour NF-kB p65 anticorps (pSer536) (ABIN730528)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Détail du produit anti-NF-kB p65 anticorps

Épitope: pSer536

Réactivité croisée: Poulet, Boeuf (Vache), Humain, Souris, Rat

Homologie: Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of(Ser536)

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-NF-kB p65 (ABIN730528)

Zhuang, Liu, Barkema, Zhou, Xu, Ur Rahman, Liu, Kastelic, Gao, Han: "Selenomethionine Suppressed TLR4/NF-κB Pathway by Activating Selenoprotein S to Alleviate ESBL Escherichia coli-Induced Inflammation in Bovine Mammary Epithelial Cells and Macrophages." dans: Frontiers in microbiology, Vol. 11, pp. 1461, (2020) (PubMed).

Liu, Li, Yang, Niu, Zhao, Zhao, Wang: "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." dans: Biomedicine & pharmacotherapy, Vol. 96, pp. 1363-1370, (2018) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." dans: Experimental animals, (2017) (PubMed).

Singh, Koduru, Carlisle, Akhter, Liu, Schroder, Brandes, Ojcius: "NADPH oxidase 4 modulates hepatic responses to lipopolysaccharide mediated by Toll-like receptor-4." dans: Scientific reports, Vol. 7, Issue 1, pp. 14346, (2017) (PubMed).

Yang, Liu, Jiang, Wang, Zhang: "Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model." dans: Frontiers in pharmacology, Vol. 8, pp. 44, (2017) (PubMed).

Fu, Shi, Song, Xia, Su, Liu, Song, Song: "Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated IKK/IκB/NF-κB signaling." dans: Environmental toxicology and pharmacology, Vol. 41, pp. 241-50, (2016) (PubMed).

Zhao, Zheng, Hu, Shen, Li: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." dans: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).

Zhang, Zhao, Yuan, Wu, Jiang, Luo, Zhao: "Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway." dans: International immunopharmacology, Vol. 38, pp. 167-74, (2016) (PubMed).

Gao, Guo, Wang, Lin, Yu, Sheng, Wu, Gong: "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." dans: Acta histochemica, Vol. 118, Issue 6, pp. 606-14, (2016) (PubMed).

Yan, Wu, Li, Meng, Dai, Zheng, Fu: "Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis rats." dans: The Journal of surgical research, Vol. 199, Issue 2, pp. 592-600, (2015) (PubMed).

Zhao, Liu, Liu, Han, Zhao: "Betulin attenuates lung and liver injuries in sepsis." dans: International immunopharmacology, Vol. 30, pp. 50-6, (2015) (PubMed).

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NFKB p65

Sujet:

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA

Background: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

ID gène: 5970

UniProt: Q04206

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins