Recombinant PKC alpha anticorps (pThr497)

N° du produit ABIN7799986

Cet anticorps anti-PKC alpha Monoclonal Lapin (Clone 23GB4955) (ABIN7799986) détecte spécifiquement PKC alpha dans WB, FACS et IF (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant PKC alpha anticorps (pThr497) (ABIN7799986)

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC alpha est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Classe de qualité: KD Validated

Clone: 23GB4955

Détail du produit anti-PKC alpha anticorps

Épitope: pThr497

Expression System: HEK-293 Cells

Fonction: KD-Validated Phospho-PKC alpha (T497) Recombinant Rabbit mAb

Réactivité croisée: Souris, Rat

Purification: Affinity Purified

Immunogène: A synthesized peptide derived from human Phospho-PKC alpha (T497)

Isotype: IgG

Information d'application

Indications d'application: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Commentaires:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C for one year.

Date de péremption: 12 months

Détails sur PKC alpha

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Autre désignation: PKC alpha

Poids moléculaire: 77 kDa

ID gène: 5578

UniProt: P17252

Pathways: Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling