Recombinant Phospholipase C gamma 1 anticorps (pTyr1253)

N° du produit ABIN7879825

Cet anticorps anti-Phospholipase C gamma 1 Monoclonal Lapin (Clone 32P05) (ABIN7879825) détecte spécifiquement Phospholipase C gamma 1 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant Phospholipase C gamma 1 anticorps (pTyr1253) (ABIN7879825)

Antigène: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Phospholipase C gamma 1 est non-conjugé

Application: Western Blotting (WB)

Clone: 32P05

Détail du produit anti-Phospholipase C gamma 1 anticorps

Épitope: pTyr1253

Fonction: Phospho-Phospholipase C gamma 1 (Tyr1253) Antibody / PLCG1

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human phospho-Phospholipase C gamma 1/PLC-gamma-1 (Y1253) was used as the immunogen for the Phospho-Phospholipase C gamma 1 (Tyr1253) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-Phospholipase C gamma 1 (Tyr1253) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-Phospholipase C gamma 1 (Tyr1253) antibody at -20oC.

Détails sur Phospholipase C gamma 1

Antigène: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Autre désignation: Phospholipase C gamma 1

Sujet: Phospho-Phospholipase C gamma 1 (Tyr1253) antibody targets phospholipase C gamma 1 (PLCG1) when phosphorylated at tyrosine 1253. PLCG1 is an essential enzyme in intracellular signal transduction, catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate. These second messengers trigger activation of protein kinase C and mobilization of intracellular calcium, respectively, initiating diverse downstream cellular responses.

Phosphorylation of PLCG1 at tyrosine 1253 is mediated by receptor tyrosine kinases and non-receptor tyrosine kinases. This phosphorylation event enhances catalytic activity and contributes to cell proliferation, differentiation, migration, and survival. Dysregulation of PLCG1 phosphorylation is implicated in oncogenesis, autoimmune disease, and vascular disorders.

Phospho-Phospholipase C gamma 1 (Tyr1253) antibody provides precise detection of the phosphorylated form, enabling researchers to evaluate signaling activation downstream of growth factor receptors such as EGFR, PDGFR, and VEGFR. It is commonly applied in studies involving cancer biology, immune receptor signaling, and angiogenesis.

This antibody is highly suitable for methods such as western blot, immunohistochemistry, immunofluorescence, and flow cytometry. By differentiating phosphorylated PLCG1 from total protein levels, it provides insight into dynamic cellular signaling events.

PLCG1 phosphorylation is especially important in cancer biology, where hyperactivation of growth factor signaling drives uncontrolled proliferation. Monitoring tyrosine 1253 phosphorylation provides mechanistic understanding of oncogenic transformation and therapeutic responses to kinase inhibitors. In immunology, PLCG1 phosphorylation regulates antigen receptor signaling, impacting T cell and B cell responses.

Phospho-Phospholipase C gamma 1 (Tyr1253) antibody from NSJ Bioreagents is a valuable reagent for researchers studying tyrosine kinase signaling, cell growth regulation, and immune function. Its phospho-specific recognition ensures accurate assessment of pathway activation in health and disease.

UniProt: P19174

Pathways: Signalisation RTK, Signalisation WNT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling