WNT2 anticorps (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290)

Details for Product anti-WNT2 Antibody No. ABIN762896, Fournisseur: Connectez-vous pour afficher
Antigène
  • CG1916
  • D-wnt-2
  • DWnt-2
  • DWnt2
  • Dm DWnt2
  • Dm-2
  • Dmel\\CG1916
  • Dwnt-2
  • Wnt
  • Wnt-2
  • dWnt2
  • wnt2
  • WNT2
  • irp
  • Xwnt2
  • wnt-2
  • Xwnt-2
  • int1l1
  • 2610510E18Rik
  • Int1l1
  • Irp
  • Mirp
  • Wnt2a
  • INT1L1
  • IRP
  • ZfWnt2
  • Wnt oncogene analog 2
  • Wnt family member 2
  • wingless-type MMTV integration site family member 2
  • wingless-type MMTV integration site family, member 2
  • Wnt2
  • wnt2
  • WNT2
Épitope
AA 240-290
14
6
3
3
2
2
2
1
1
1
1
Reactivité
Chien, Humain, Souris, Rat (Rattus)
64
37
31
18
4
4
4
4
4
4
3
2
2
Hôte
Lapin
66
Clonalité
Polyclonal
Conjugué
Cet anticorp WNT2 est non-conjugé
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
41
13
12
10
5
1
1
1
Options
Fournisseur
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Supplier Product No.
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'Independent Validation' signe
Numéro du lot 131105
Application validée Immunohistochemistry
Contrôle positif Human glioma tissue
Contrôle négative Human brain
Conclusion Signal was detected in positive control tissue and not in negative control tissue.
Anticorps primaire
Contrôle isotypique
Anticorps secondaire
Contrôles
  • Positive control: Human Glioma (specimen known to contain the target protein) from HTSR’s Human Tissue Bank.
  • Negative Control: Human Normal Brain (specimen known to not contain the target protein) from HTSR’s Human Tissue Bank.
  • Primary antibody isotype control: Human Glioma treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Human Glioma treated with secondary antibody only (no primary antibody).
Protocole
  • Immunohistochemistry was performed by hand.
  • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
  • Sections were rinsed in de-ionized water for 5 min at RT.
  • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
  • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
  • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
  • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
  • Sections were washed x 1 in Distilled Water.
  • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were blued in Ammonium Hydroxide for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
Notes
  • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
  • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
Validation Images
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 1: Human brain glioma tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 2: Human normal brain tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 3: Human brain glioma tissue stained with isotype control antibody (brown) and...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 4: Human brain glioma tissue stained with secondary only (brown) and counterst...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 5: Human brain tissue with Parkinson's morphology stained with anti-Wnt2 (brow...
Immunogène KLH conjugated synthetic peptide derived from human WNT2
Isotype IgG
Purification Purified by Protein A.
Autre désignation WNT2 (WNT2 Antibody Extrait)
Sujet

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Subcellular location: Secreted

Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2
ID gène 7472
UniProt P09544
Pathways Signalisation WNT
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Formalin-fixed and paraffin embedded human placenta labeled with Anti-WNT2 Polyclonal...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown i...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded ca...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Mouse brain lysates probed with Anti-WNT2 Polyclonal Antibody, Unconjugated at 1:500...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Image provided by the Independent Validation Program (badge number 29808). Lane 1: A5...
Produit citée dans: Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." dans: PLoS ONE, Vol. 9, Issue 1, pp. e83995, 2014 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Dog (Canine)).

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