CD45 anticorps

N° du produit ABIN775710

Détail du produit anti-CD45 anticorps

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificité: The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified by protein-G affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: human peripheral blood leukocytes

Clone: HI30

Isotype: IgG1 kappa

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1 μg/mL, non-reducing conditions, band around 200-250 kDa, positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate, non-reducing conditions, 6 % separating gel.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD45 (ABIN775710)

Song, Ara, Wu, Woo, Reynolds, Seeger, DeClerck, Thiele, Sposto, Metelitsa: "Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma." dans: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2702-12, (2007) (PubMed).

Leone, Rutella, Bonanno, Abbate, Rebuzzi, Giovannini, Lombardi, Galiuto, Liuzzo, Andreotti, Lanza, Contemi, Leone, Crea: "Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function." dans: European heart journal, Vol. 26, Issue 12, pp. 1196-204, (2005) (PubMed).

Yamada, Zhu, Saxon, Zhang: "CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase." dans: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28830-5, (2002) (PubMed).

Détails sur CD45

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: CD45 (PTPRC Produits)

Synonymes: anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc

Sujet: Protein tyrosine phosphatase receptor type C,CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10 % of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.,LCA, T200, LY5, B220, GP180, TPC

ID gène: 5788

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling