RANKL anticorps (AA 143-316)
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- Antigène Voir toutes RANKL (TNFSF11) Anticorps
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
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Épitope
- AA 143-316
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Reactivité
- Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RANKL est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Specificité
- Anti-RANK L antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97-99% homology, there is a chance of cross-reactivity to RANKL from rat, based on 89-94% homology, a chance of cross-reactivity to horse RANKL, on 87-93% homology, to human RANKL-2 and RANKL isoform 1 and to orangutan RANKL, and on 86-93% homology, a chance of cross-reactivity to dog, cattle, sheep, pig, chimpanzee, and macaque RANKL. Cross-reactivity with RANKL from other sources has not been determined.
- Attributs du produit
- Anti-RANK L antibody is useful in studying the regulating NF-kappaB activation. Secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE, ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form.
- Purification
- purified
- Stérilité
- Sterile filtered
- Immunogène
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This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
Immunogen Type: RecombinantProtein - Isotype
- IgG
- Top Product
- Discover our top product TNFSF11 Anticorps primaire
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- Indications d'application
- This purified antibody has been tested for use in ELISA and western blotting.. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kDa in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.
- Commentaires
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Gene Name: Tnfsf11
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Agent conservateur
- Without preservative
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.
- Date de péremption
- 6 months
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- Antigène
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
- Abstract
- TNFSF11 Produits
- Synonymes
- anticorps CD254, anticorps ODF, anticorps OPGL, anticorps OPTB2, anticorps RANKL, anticorps TRANCE, anticorps hRANKL2, anticorps sOdf, anticorps TNFSF11, anticorps Ly109l, anticorps OPG, anticorps Trance, anticorps cd254, anticorps odf, anticorps opgl, anticorps optb2, anticorps rankl, anticorps sodf, anticorps tnfsf11, anticorps trance, anticorps TNF superfamily member 11, anticorps tumor necrosis factor (ligand) superfamily, member 11, anticorps TNF receptor superfamily member 11b, anticorps TNF superfamily member 11 L homeolog, anticorps TNFSF11, anticorps Tnfsf11, anticorps TNFRSF11B, anticorps tnfsf11.L
- Sujet
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Anti-RANK L antibody is useful in studying the regulating NF-κB activation. Secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE; ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form.
Synonyms: Receptor activator of nuclear factor kappa B, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNF11, TNFRSF11, Osteoprotegerin ligand, OPG, Osteoclast differentiation factor, ODF, CD254 - ID gène
- 21943
- NCBI Accession
- NP_035743
- UniProt
- O35235
- Pathways
- Signalisation NF-kappaB
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