TRKB Kit ELISA

N° du produit ABIN1112664

Détail du produit TRKB Kit ELISA

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.8-6000 pg/mL

Seuil minimal de détection: 93.8 pg/mL

Application: ELISA

Fonction: For quantitative detection of mouse TrkB in tissue lysates or cell culture supernates.

Type d'échantillon: Tissue Lysate, Cell Culture Supernatant

Analytical Method: Quantitative

Sensibilité: < 5 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Mouse trkB antibody 2. Lyophilized Mouse trkB standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse trkB antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TrkB polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TrkB polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the TrkB amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of TrkB can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids, cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Autre désignation: TRKB (NTRK2 Produits)

Synonymes: trkb Kit ELISA, trkb-b Kit ELISA, xtrkb Kit ELISA, si:ch211-220b11.1 Kit ELISA, trkB2 Kit ELISA, NTRK2 Kit ELISA, ntrk2 Kit ELISA, gp145-trkb Kit ELISA, TRKB Kit ELISA, GP145-TrkB Kit ELISA, trk-B Kit ELISA, AI848316 Kit ELISA, C030027L06Rik Kit ELISA, Tkrb Kit ELISA, trkB Kit ELISA, RATTRKB1 Kit ELISA, TRKB1 Kit ELISA, Trk-B Kit ELISA, TrkB Kit ELISA, neurotrophic receptor tyrosine kinase 2 Kit ELISA, neurotrophic receptor tyrosine kinase 2 L homeolog Kit ELISA, neurotrophic tyrosine kinase, receptor, type 2b Kit ELISA, neurotrophic receptor tyrosine kinase 2 S homeolog Kit ELISA, neurotrophic tyrosine kinase, receptor, type 2 Kit ELISA, NTRK2 Kit ELISA, ntrk2.L Kit ELISA, ntrk2b Kit ELISA, ntrk2.S Kit ELISA, ntrk2 Kit ELISA, Ntrk2 Kit ELISA

Sujet: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene. The TrkB receptor is part of the large family of receptor tyrosine kinases, it is located at the cellular membrane, and is activated by binding of a ligand to the receptor's extracellular domain. TrkB is the high affinity catalytic receptor for several neurotrophins, which are small protein growth factors that induce the survival and differentiation of distinct cell populations.

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport