TNFRSF13C Kit ELISA

N° du produit ABIN1706072

Détail du produit TNFRSF13C Kit ELISA

Antigène: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Épitope: AA 7-71

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78-5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF13C/BAFFR

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: S7-A71

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: S7-A71

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.

Plaque: Pre-coated

Protocole: human TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF13C has been precoated onto 96-well plates. Standards(NSO, S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF13C amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human TNFRSF13C standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF13C standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 643, Standard deviation: 29.6, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 1604, Standard deviation: 57.75, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 3262, Standard deviation: 169.6, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 745, Standard deviation: 43.21, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 1862, Standard deviation: 91.3, CV(%): 4.9
• Sample 3: n=24, Mean(pg/ml): 3657, Standard deviation: 234.1, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Autre désignation: TNFRSF13C (TNFRSF13C Produits)

Synonymes: RGD1560810 Kit ELISA, TNFRSF13C Kit ELISA, BAFF-R Kit ELISA, BAFFR Kit ELISA, BROMIX Kit ELISA, CD268 Kit ELISA, CVID4 Kit ELISA, prolixin Kit ELISA, 2010006P15Rik Kit ELISA, Baffr Kit ELISA, Bcmd Kit ELISA, Bcmd-1 Kit ELISA, Bcmd1 Kit ELISA, Lvis22 Kit ELISA, TNF receptor superfamily member 13C Kit ELISA, uncharacterized LOC746611 Kit ELISA, tumor necrosis factor receptor superfamily, member 13c Kit ELISA, TNFRSF13C Kit ELISA, Tnfrsf13c Kit ELISA, LOC746611 Kit ELISA

Sujet:

Protein Function: B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. .

Background: Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Synonyms: Tumor necrosis factor receptor superfamily member 13C,B-cell-activating factor receptor,BAFF receptor,BAFF-R,BLyS receptor 3,CD268,TNFRSF13C,BAFFR, BR3,

Full Gene Name: Tumor necrosis factor receptor superfamily member 13C

Cellular Localisation: Membrane, Single-pass type III membrane protein.

ID gène: 115650

UniProt: Q96RJ3

Pathways: Signalisation NF-kappaB