TNFRSF13C Kit ELISA

N° du produit ABIN2702844

Détail du produit TNFRSF13C Kit ELISA

Antigène: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 28-7.000 pg/mL

Seuil minimal de détection: 28 pg/mL

Application: ELISA

Fonction: Mouse BAFF R ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following mouse cytokines tested: BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon

Sensibilité: 28 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Autre désignation: BAFF R (TNFRSF13C Produits)

Synonymes: RGD1560810 Kit ELISA, TNFRSF13C Kit ELISA, BAFF-R Kit ELISA, BAFFR Kit ELISA, BROMIX Kit ELISA, CD268 Kit ELISA, CVID4 Kit ELISA, prolixin Kit ELISA, 2010006P15Rik Kit ELISA, Baffr Kit ELISA, Bcmd Kit ELISA, Bcmd-1 Kit ELISA, Bcmd1 Kit ELISA, Lvis22 Kit ELISA, TNF receptor superfamily member 13C Kit ELISA, uncharacterized LOC746611 Kit ELISA, tumor necrosis factor receptor superfamily, member 13c Kit ELISA, TNFRSF13C Kit ELISA, Tnfrsf13c Kit ELISA, LOC746611 Kit ELISA

Sujet: Gene Names: Tnfrsf13c Baffr Bcmd Br3
Protein names: Tumor necrosis factor receptor superfamily member 13C (B-cell maturation defect) (B-cell-activating factor receptor) (BAFF receptor) (BAFF-R) (BLyS receptor 3) (CD antigen CD268)

ID gène: 72049

UniProt: Q9D8D0

Pathways: Signalisation NF-kappaB