TNFRSF13C Kit ELISA

N° du produit ABIN6951091

Détail du produit TNFRSF13C Kit ELISA

Antigène: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human BAFF R ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Autre désignation: BAFF R (TNFRSF13C Produits)

Synonymes: RGD1560810 Kit ELISA, TNFRSF13C Kit ELISA, BAFF-R Kit ELISA, BAFFR Kit ELISA, BROMIX Kit ELISA, CD268 Kit ELISA, CVID4 Kit ELISA, prolixin Kit ELISA, 2010006P15Rik Kit ELISA, Baffr Kit ELISA, Bcmd Kit ELISA, Bcmd-1 Kit ELISA, Bcmd1 Kit ELISA, Lvis22 Kit ELISA, TNF receptor superfamily member 13C Kit ELISA, uncharacterized LOC746611 Kit ELISA, tumor necrosis factor receptor superfamily, member 13c Kit ELISA, TNFRSF13C Kit ELISA, Tnfrsf13c Kit ELISA, LOC746611 Kit ELISA

ID gène: 115650

UniProt: Q96RJ3

Pathways: Signalisation NF-kappaB