IL-3 Kit ELISA
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- Antigène Voir toutes IL-3 Kits ELISA
- IL-3 (Interleukin 3 (IL-3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Application
- ELISA
- Fonction
- Human Interleukin 3 (IL-3) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 3 (IL-3) concentrations in cell culture supernates, serum, and plasma.
- Type d'échantillon
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensibilité
- 15 pg/ml
- Attributs du produit
- Human Interleukin 3 (IL-3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Volume d'échantillon
- 100 μL
- Durée du test
- 3.5 h
- Plaque
- Pre-coated
- Protocole
- Human Interleukin 3 (IL-3) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-3 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-3 is added to the wells and binds to the combination of capture antibody- IL-3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-3 standard dilutions and IL-3 sample concentration determined.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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- Antigène Voir toutes IL-3 Kits ELISA
- IL-3 (Interleukin 3 (IL-3))
- Autre désignation
- IL-3 (IL-3 Produits)
- Synonymes
- BPA Kit ELISA, Csfmu Kit ELISA, HCGF Kit ELISA, Il-3 Kit ELISA, MCGF Kit ELISA, PSF Kit ELISA, IL-3 Kit ELISA, MULTI-CSF Kit ELISA, interleukin 3 Kit ELISA, interleukin 3 (colony-stimulating factor, multiple) Kit ELISA, Il3 Kit ELISA, IL3 Kit ELISA
- Pathways
- Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy
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