Midkine Kit ELISA

N° du produit ABIN2703330

Détail du produit Midkine Kit ELISA

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.12-30 ng/mL

Seuil minimal de détection: 0.12 ng/mL

Application: ELISA

Fonction: Human Midkine ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1

Sensibilité: 0.12 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Autre désignation: Midkine (MDK Produits)

Synonymes: ARAP Kit ELISA, MK Kit ELISA, NEGF2 Kit ELISA, Mek Kit ELISA, MK-B Kit ELISA, PTF-alpha-1 Kit ELISA, mdk Kit ELISA, midkine Kit ELISA, negf2 Kit ELISA, RIHB Kit ELISA, cb518 Kit ELISA, fb79b05 Kit ELISA, fj33b02 Kit ELISA, fj48a12 Kit ELISA, mdk2 Kit ELISA, plei2 Kit ELISA, wu:fb79b05 Kit ELISA, wu:fj33b02 Kit ELISA, wu:fj48a12 Kit ELISA, MK-A Kit ELISA, PTF-alpha-2 Kit ELISA, X-PTF-alpha1 Kit ELISA, XMK Kit ELISA, mdk-a Kit ELISA, mdk-b Kit ELISA, midkine Kit ELISA, midkine L homeolog Kit ELISA, midkine (neurite growth-promoting factor 2) Kit ELISA, midkine b Kit ELISA, midkine S homeolog Kit ELISA, MDK Kit ELISA, Mdk Kit ELISA, mdk.L Kit ELISA, mdkb Kit ELISA, mdk.S Kit ELISA

Sujet: Gene Names: MDK MK1 NEGF2
Protein names: Midkine (MK) (Amphiregulin-associated protein) (ARAP) (Midgestation and kidney protein) (Neurite outgrowth-promoting factor 2) (Neurite outgrowth-promoting protein)

ID gène: 4192

UniProt: P21741

Pathways: Signalisation RTK, M Phase, Skeletal Muscle Fiber Development