SERPINA4 Kit ELISA

N° du produit ABIN2703452

Détail du produit SERPINA4 Kit ELISA

Antigène: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.12-35 ng/mL

Seuil minimal de détection: 0.12 ng/mL

Application: ELISA

Fonction: Human SERPINA4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human Serpin A4. Other species not determined.

Sensibilité: 0.12 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples10,000 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Autre désignation: Serpin A4 (SERPINA4 Produits)

Synonymes: KLKR Kit ELISA, Klk6 Kit ELISA, hK1 Kit ELISA, KAL Kit ELISA, KLST Kit ELISA, KST Kit ELISA, PI-4 Kit ELISA, PI4 Kit ELISA, kallistatin Kit ELISA, SERPINA4 Kit ELISA, DKFZp469E1737 Kit ELISA, KALps Kit ELISA, Klst Kit ELISA, kallikrein 1 Kit ELISA, serpin family A member 4 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 Kit ELISA, KLK1 Kit ELISA, SERPINA4 Kit ELISA, Serpina4 Kit ELISA, SPIA4 Kit ELISA, Serpina4-ps1 Kit ELISA

Sujet: Gene Names: SERPINA4 KST PI4
Protein names: Kallistatin (Kallikrein inhibitor) (Peptidase inhibitor 4) (PI-4) (Serpin A4)

ID gène: 5267

UniProt: P29622