TRAIL Kit ELISA

N° du produit ABIN2703528

Détail du produit TRAIL Kit ELISA

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2-600 pg/mL

Seuil minimal de détection: 2 pg/mL

Application: ELISA

Fonction: Mouse TRAIL (TNFSF10) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects mouse TRAIL. Other species not determined.

Sensibilité: 2 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour TRAIL Kit ELISA (ABIN2703528)

Ni, Wu, Peterts, Yamamoto, Qing, Nakanishi: "The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 36, pp. 12488-501, (2015) (PubMed).

Détail du antigène

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: TRAIL (TNFSF10 Produits)

Synonymes: APO2L Kit ELISA, Apo-2L Kit ELISA, CD253 Kit ELISA, TL2 Kit ELISA, TRAIL Kit ELISA, A330042I21Rik Kit ELISA, AI448571 Kit ELISA, APO-2L Kit ELISA, Ly81 Kit ELISA, Trail Kit ELISA, tl2 Kit ELISA, apo2l Kit ELISA, cd253 Kit ELISA, trail Kit ELISA, apo-2l Kit ELISA, trail1 Kit ELISA, xtrail1 Kit ELISA, tnfsf10l2 Kit ELISA, zgc:92320 Kit ELISA, TNLG6A Kit ELISA, TNF superfamily member 10 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 10 Kit ELISA, tumor necrosis factor superfamily member 10 Kit ELISA, TNF superfamily member 10 S homeolog Kit ELISA, TNFSF10 Kit ELISA, Tnfsf10 Kit ELISA, tnfsf10.S Kit ELISA, tnfsf10 Kit ELISA

Sujet: Gene Names: Tnfsf10 Trail
Protein names: Tumor necrosis factor ligand superfamily member 10 (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)

ID gène: 22035

UniProt: P50592

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity