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p53 Kit ELISA

TP53 Reactivité: Humain, Rat, Souris pSer15 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2748489
  • Antigène Voir toutes p53 (TP53) Kits ELISA
    p53 (TP53) (Tumor Protein P53 (TP53))
    Épitope
    pSer15
    Reactivité
    • 31
    • 17
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Rat, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Phospho-P53 (S15) ELISA Kit. This assay semi-quantitatively measures phophorylated P53 (Ser15) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human Phospho-P53 (pSer15).
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes p53 (TP53) Kits ELISA
    p53 (TP53) (Tumor Protein P53 (TP53))
    Autre désignation
    P53 (TP53 Produits)
    Synonymes
    BCC7 Kit ELISA, LFS1 Kit ELISA, P53 Kit ELISA, TRP53 Kit ELISA, p53 Kit ELISA, Trp53 Kit ELISA, brp53 Kit ELISA, drp53 Kit ELISA, etID22686.5 Kit ELISA, fb40d06 Kit ELISA, wu:fb40d06 Kit ELISA, zgc:111919 Kit ELISA, Tp53 Kit ELISA, bbl Kit ELISA, bfy Kit ELISA, bhy Kit ELISA, p44 Kit ELISA, tp53 Kit ELISA, Xp53 Kit ELISA, TP53 Kit ELISA, tumor protein p53 Kit ELISA, cellular tumor antigen p53 Kit ELISA, transformation related protein 53 Kit ELISA, tumor protein p53 L homeolog Kit ELISA, TP53 Kit ELISA, CpipJ_CPIJ002758 Kit ELISA, Tp53 Kit ELISA, tp53 Kit ELISA, Trp53 Kit ELISA, tp53.L Kit ELISA
    Sujet
    P53 (Tumor protein p53, cellular tumor antigen p53, phosphoprotein p53, tumor suppressor p53) phosphorylated at Serine-15
    ID gène
    7157
    UniProt
    P04637
    Pathways
    Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg
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