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TNFSF4 Kit ELISA
TNFSF4
Reactivité: Humain
Colorimetric
Sandwich ELISA
61-15.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884063
Détail du produit TNFSF4 Kit ELISA
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Antigène
Voir toutes TNFSF4 Kits ELISA
TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
Reactivité
Toutes les réactivités sur TNFSF4 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
61-15.000 pg/mL
Seuil minimal de détection
61 pg/mL
Application
ELISA
Fonction
Human OX40 Ligand (TNFSF4) ELISA Kit for serum, plasma, and cell culture supernatant samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC-1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1, Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TNFSF4 Kits ELISA
TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
Autre désignation
OX40 Ligand///TNFSF4 (TNFSF4 Produits )
Synonymes
TNFSF4 Kit ELISA, OX40L Kit ELISA, TNFRSF4 Kit ELISA, CD134L Kit ELISA, CD252 Kit ELISA, GP34 Kit ELISA, OX-40L Kit ELISA, OX4OL Kit ELISA, TXGP1 Kit ELISA, Ath-1 Kit ELISA, Ath1 Kit ELISA, Ox40l Kit ELISA, Txgp1l Kit ELISA, gp34 Kit ELISA, TNF superfamily member 4 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 4 Kit ELISA, TNFSF4 Kit ELISA, Tnfsf4 Kit ELISA
ID gène
7292
UniProt
P23510
Pathways
Cellular Response to Molecule of Bacterial Origin , Regulation of Leukocyte Mediated Immunity , Positive Regulation of Immune Effector Process , Production of Molecular Mediator of Immune Response , Activated T Cell Proliferation , Cancer Immune Checkpoints
Vus récemment
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