SFRP1 Kit ELISA

N° du produit ABIN4884499

Détail du produit SFRP1 Kit ELISA

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Human sFRP-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human sFRP-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples3 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Autre désignation: sFRP-1 (SFRP1 Produits)

Synonymes: FRP Kit ELISA, FRP-1 Kit ELISA, FRP1 Kit ELISA, FrzA Kit ELISA, SARP2 Kit ELISA, 2210415K03Rik Kit ELISA, AW011917 Kit ELISA, AW107218 Kit ELISA, AW742929 Kit ELISA, sFRP-1 Kit ELISA, CSFRP1 Kit ELISA, SFRP1 Kit ELISA, XfrzA Kit ELISA, frp Kit ELISA, frp-1 Kit ELISA, frp1 Kit ELISA, frzA Kit ELISA, sarp2 Kit ELISA, sfrp1 Kit ELISA, LOC100231868 Kit ELISA, Srfp1a Kit ELISA, wu:fd19b09 Kit ELISA, secreted frizzled related protein 1 Kit ELISA, secreted frizzled-related protein 1 Kit ELISA, Secreted frizzled-related protein 1 Kit ELISA, secreted frizzled-related protein 1a Kit ELISA, secreted frizzled-related protein 1b Kit ELISA, SFRP1 Kit ELISA, Sfrp1 Kit ELISA, sfrp1 Kit ELISA, sfrp1a Kit ELISA, sfrp1b Kit ELISA

ID gène: 6422

UniProt: Q8N474

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation