SFRP1 Kit ELISA
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- Antigène Voir toutes SFRP1 Kits ELISA
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.78-50 ng/mL
- Seuil minimal de détection
- 0.78 ng/mL
- Application
- ELISA
- Fonction
- Human sFRP-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human sFRP-1.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples3 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes SFRP1 Kits ELISA
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
- Autre désignation
- sFRP-1 (SFRP1 Produits)
- Synonymes
- FRP Kit ELISA, FRP-1 Kit ELISA, FRP1 Kit ELISA, FrzA Kit ELISA, SARP2 Kit ELISA, 2210415K03Rik Kit ELISA, AW011917 Kit ELISA, AW107218 Kit ELISA, AW742929 Kit ELISA, sFRP-1 Kit ELISA, CSFRP1 Kit ELISA, SFRP1 Kit ELISA, XfrzA Kit ELISA, frp Kit ELISA, frp-1 Kit ELISA, frp1 Kit ELISA, frzA Kit ELISA, sarp2 Kit ELISA, sfrp1 Kit ELISA, LOC100231868 Kit ELISA, Srfp1a Kit ELISA, wu:fd19b09 Kit ELISA, secreted frizzled related protein 1 Kit ELISA, secreted frizzled-related protein 1 Kit ELISA, Secreted frizzled-related protein 1 Kit ELISA, secreted frizzled-related protein 1a Kit ELISA, secreted frizzled-related protein 1b Kit ELISA, SFRP1 Kit ELISA, Sfrp1 Kit ELISA, sfrp1 Kit ELISA, sfrp1a Kit ELISA, sfrp1b Kit ELISA
- ID gène
- 6422
- UniProt
- Q8N474
- Pathways
- Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
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