p53 Kit ELISA

N° du produit ABIN4885890

Détail du produit p53 Kit ELISA

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.328-80 ng/mL

Seuil minimal de détection: 0.328 ng/mL

Application: ELISA

Fonction: Rat p53 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Rat p53.

Sensibilité: 0.32 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53 (TP53 Produits)

Synonymes: BCC7 Kit ELISA, LFS1 Kit ELISA, P53 Kit ELISA, TRP53 Kit ELISA, p53 Kit ELISA, Trp53 Kit ELISA, brp53 Kit ELISA, drp53 Kit ELISA, etID22686.5 Kit ELISA, fb40d06 Kit ELISA, wu:fb40d06 Kit ELISA, zgc:111919 Kit ELISA, Tp53 Kit ELISA, bbl Kit ELISA, bfy Kit ELISA, bhy Kit ELISA, p44 Kit ELISA, tp53 Kit ELISA, Xp53 Kit ELISA, TP53 Kit ELISA, tumor protein p53 Kit ELISA, cellular tumor antigen p53 Kit ELISA, transformation related protein 53 Kit ELISA, tumor protein p53 L homeolog Kit ELISA, TP53 Kit ELISA, CpipJ_CPIJ002758 Kit ELISA, Tp53 Kit ELISA, tp53 Kit ELISA, Trp53 Kit ELISA, tp53.L Kit ELISA

ID gène: 24842

UniProt: P10361

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg