IL12 Kit ELISA

N° du produit ABIN5068331

Détail du produit IL12 Kit ELISA

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum, Tissue Samples

Sensibilité: 7 pg/mL

Attributs du produit: IL-12 p70 ELISA Kit (Rat) (Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35kD subunit, CLMF p35, IL-12 subunit p35, NK cell stimulatory factor chain 1, IL12A, NKSF1, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40kD)

Ingrédients:

• Aluminium pouches with a Microwell Plate: 8x12
• Standard: 2xVial
• Biotinylated antibody: 2xVial
• Streptavidin-HRP: 2xVial
• Assay Buffer (25ml/Vial): 2xVial
• Wash Buffer Concentrate 20x (30ml/Vial): 1xVial
• TMB Substrate Solution: 1x12ml
• Stop Solution (12ml/Vial): 1xVial
• Plate Covers- Adhesive strips: 3xstrips
• Instruction: 1x

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• This assay employs the Sandwich immunoassay technique. An anti-r IL-12 p70 monoclonal coating antibody is adsorbed onto microwells. IL-12 p70 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-r IL-12 p70 antibody is added and binds to IL-12 p70 captured by the first antibody. Following incubation unbound Biotinylated anti-r IL-12 p70 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-r IL-12 p70 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-12 p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12 p70 standard dilutions and IL-12 p70 sample concentration determined.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12 p70 (IL12 Produits)

Synonymes: CLMF Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12 Kit ELISA, LOC100217488 Kit ELISA, IL-12p35 Kit ELISA, Il-12a Kit ELISA, Ll12a Kit ELISA, p35 Kit ELISA, IL12p40 Kit ELISA, IL12B Kit ELISA, IL-12 p35 Kit ELISA, interleukin 12B Kit ELISA, interleukin 12A Kit ELISA, interleukin 12b Kit ELISA, interleukin 12a Kit ELISA, Interleukin 12 level Kit ELISA, IL12B Kit ELISA, IL12A Kit ELISA, Il12b Kit ELISA, Il12a Kit ELISA, IL12 Kit ELISA, il12a Kit ELISA

Sujet: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome