AGT Kit ELISA (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8))

Details for Product AGT Kit ELISA No. ABIN5651773
Antigène
Reactivité
Chien
Alternatives
Kits with alternative reactivity to:
6
5
5
4
2
2
2
2
2
1
1
1
Méthode de détection
Colorimetric
Type de méthode
Competition ELISA
Gamme de detection
24.69 pg/mL - 2000 pg/mL
Seuil minimal de détection
24.69 pg/mL
Application
ELISA
Options
Type d'échantillon Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This assay has high sensitivity and excellent specificity for detection of Angiotensin I (AngI). No significant cross-reactivity or interference between Angiotensin I (AngI) and analogues was observed.
Sensibilité 9.28 pg/mL
Antigène
Autre désignation Angiotensin I (AGT ELISA Kit Extrait)
Sujet

Gene Name: Angiotensin I

Gene Aliases: Ang-I, Angiotensin-1

Pathways Signalistation JAK/STAT, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity
Commentaires

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test 2 h
Plaque Pre-coated
Protocole This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin I (AngI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin I (AngI) and unlabeled Angiotensin I (AngI) (Standards or samples) with the pre-coated antibody specific to Angiotensin I (AngI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin I (AngI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin I (AngI) in the sample.
Précision du teste Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I (AngI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I (AngI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Conseil sur la manipulation The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Stock 4 °C,-20 °C
Stockage commentaire -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Date de péremption 4-8 months