AGT Kit CLIA

N° du produit ABIN5651811

Détail du produit AGT Kit CLIA

Antigène: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Angiotensinogen (AGT). No significant cross-reactivity or interference between Angiotensinogen (AGT) and analogues was observed.

Sensibilité: 0.03 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Angiotensinogen (AGT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensinogen (AGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensinogen (AGT) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Autre désignation: Angiotensinogen (AGT Produits)

Synonymes: ANHU Kit CLIA, SERPINA8 Kit CLIA, AI265500 Kit CLIA, AngI Kit CLIA, AngII Kit CLIA, Aogen Kit CLIA, Serpina8 Kit CLIA, ANRT Kit CLIA, Ang Kit CLIA, PAT Kit CLIA, wu:fb62f06 Kit CLIA, wu:fj87b02 Kit CLIA, zgc:111892 Kit CLIA, AGT Kit CLIA, angt Kit CLIA, ANGT Kit CLIA, angiotensinogen Kit CLIA, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Kit CLIA, AGT Kit CLIA, Agt Kit CLIA, agt Kit CLIA

Sujet:

Gene Name: Angiotensinogen

Gene Aliases: ANHU, SERPINA8, Angiotensin, Renin Substrate, Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

UniProt: P11859

Pathways: Signalistation JAK/STAT, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity