BMPR1B Kit ELISA

N° du produit ABIN5652299

Détail du produit BMPR1B Kit ELISA

Antigène: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1B (BMPR1B). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1B (BMPR1B) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1B (BMPR1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1B (BMPR1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1B (BMPR1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein Receptor 1B (BMPR1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1B (BMPR1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1B (BMPR1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Autre désignation: Bone Morphogenetic Protein Receptor 1B (BMPR1B Produits)

Synonymes: ALK6 Kit ELISA, BMPR-IB Kit ELISA, FecB Kit ELISA, alk-6 Kit ELISA, alk6 Kit ELISA, cdw293 Kit ELISA, alk6b Kit ELISA, zgc:172219 Kit ELISA, BMPR1B Kit ELISA, AI385617 Kit ELISA, ALK-6 Kit ELISA, AV355320 Kit ELISA, Acvrlk6 Kit ELISA, Alk6 Kit ELISA, BMPR-1B Kit ELISA, CFK-43a Kit ELISA, SKR6 Kit ELISA, BR1b Kit ELISA, alk6tr Kit ELISA, bmpr1b Kit ELISA, zALK-6 Kit ELISA, zgc:92220 Kit ELISA, CDw293 Kit ELISA, BMP15 Kit ELISA, BMPRIB Kit ELISA, RPK-1 Kit ELISA, bone morphogenetic protein receptor type 1B Kit ELISA, bone morphogenetic protein receptor, type IBb Kit ELISA, bone morphogenetic protein receptor, type 1B Kit ELISA, bone morphogenetic protein receptor, type IBa Kit ELISA, BMPR1B Kit ELISA, bmpr1b Kit ELISA, bmpr1bb Kit ELISA, Bmpr1b Kit ELISA, bmpr1ba Kit ELISA

Sujet:

Gene Name: Bone Morphogenetic Protein Receptor 1B

Gene Aliases: CDw293, CD293, BMPR1-B, ALK6