GLUT1 Kit ELISA
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- Antigène Voir toutes GLUT1 (SLC2A1) Kits ELISA
- GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 1.25 ng/mL - 80 ng/mL
- Seuil minimal de détection
- 1.25 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Glucose Transporter 1 (GLUT1). No significant cross-reactivity or interference between Glucose Transporter 1 (GLUT1) and analogues was observed.
- Sensibilité
- 0.48 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 1 (GLUT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose Transporter 1 (GLUT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 1 (GLUT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 1 (GLUT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 1 (GLUT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 1 (GLUT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes GLUT1 (SLC2A1) Kits ELISA
- GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))
- Autre désignation
- Glucose Transporter 1 (SLC2A1 Produits)
- Synonymes
- DYT17 Kit ELISA, DYT18 Kit ELISA, DYT9 Kit ELISA, EIG12 Kit ELISA, GLUT Kit ELISA, GLUT1 Kit ELISA, GLUT1DS Kit ELISA, HTLVR Kit ELISA, PED Kit ELISA, Glut-1 Kit ELISA, Glut1 Kit ELISA, GLUTB Kit ELISA, GTG1 Kit ELISA, Gtg3 Kit ELISA, RATGTG1 Kit ELISA, SLC2A1 Kit ELISA, GLT1 Kit ELISA, GLUCOSE TRANSPORTER 1 Kit ELISA, T21H19.70 Kit ELISA, T21H19_70 Kit ELISA, plastidic GLC translocator Kit ELISA, glut1 Kit ELISA, ped Kit ELISA, slc2a1 Kit ELISA, slc2a14 Kit ELISA, solute carrier family 2 member 1 Kit ELISA, solute carrier family 2 (facilitated glucose transporter), member 1 Kit ELISA, plastidic GLC translocator Kit ELISA, solute carrier family 2 (facilitated glucose transporter), member 1 L homeolog Kit ELISA, SLC2A1 Kit ELISA, Slc2a1 Kit ELISA, PGLCT Kit ELISA, slc2a1.L Kit ELISA
- Sujet
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Gene Name: Glucose Transporter 1
Gene Aliases: SLC2A1, SLC2-A1, GLUT, Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter, Glucose transporter type 1, erythrocyte/brain, HepG2 glucose transporter
- Pathways
- Sensory Perception of Sound, Dicarboxylic Acid Transport, L'effet Warburg
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