GSK3 alpha Kit ELISA

N° du produit ABIN5654752

Le kit ELISA GSK3 alpha (ABIN5654752) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat GSK3 alpha.

Aperçu rapide pour GSK3 alpha Kit ELISA (ABIN5654752)

Antigène: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit GSK3 alpha Kit ELISA

Seuil minimal de détection: 0.625 ng/mL

Fonction: Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit for detecting GSK3a in tissue homogenates, cell lysates

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glycogen Synthase Kinase 3 Alpha (GSK3a). No significant cross-reactivity or interference between Glycogen Synthase Kinase 3 Alpha (GSK3a) and analogues was observed.

Sensibilité: 0.229 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Synthase Kinase 3 Alpha (GSK3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Synthase Kinase 3 Alpha (GSK3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Synthase Kinase 3 Alpha (GSK3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycogen Synthase Kinase 3 Alpha (GSK3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycogen Synthase Kinase 3 Alpha (GSK3a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycogen Synthase Kinase 3 Alpha (GSK3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Autre désignation: Glycogen Synthase Kinase 3 Alpha

Sujet: Gene Aliases: Serine/threonine-protein kinase GSK3A

ID gène: 50686

UniProt: P18265

Pathways: Signalisation PI3K-Akt, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, L'effet Warburg