HFE2 Kit ELISA

N° du produit ABIN5655034

Détail du produit HFE2 Kit ELISA

Antigène: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hemojuvelin (HJV). No significant cross-reactivity or interference between Hemojuvelin (HJV) and analogues was observed.

Sensibilité: 0.256 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemojuvelin (HJV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemojuvelin (HJV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemojuvelin (HJV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemojuvelin (HJV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemojuvelin (HJV) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemojuvelin (HJV) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Autre désignation: Hemojuvelin (HFE2 Produits)

Synonymes: HFE2A Kit ELISA, HJV Kit ELISA, JH Kit ELISA, RGMC Kit ELISA, HFE2 Kit ELISA, DKFZp468F2322 Kit ELISA, 2310035L15Rik Kit ELISA, 5230400G09Rik Kit ELISA, AI414844 Kit ELISA, AI789733 Kit ELISA, DL-M Kit ELISA, Rgmc Kit ELISA, hemojuvelin Kit ELISA, RGMr Kit ELISA, hjv Kit ELISA, id:ibd3464 Kit ELISA, wu:fb38f10 Kit ELISA, zgc:136698 Kit ELISA, hemochromatosis type 2 (juvenile) Kit ELISA, hemochromatosis type 2 Kit ELISA, HFE2 Kit ELISA, Hfe2 Kit ELISA, hfe2 Kit ELISA

Sujet:

Gene Name: Hemojuvelin

Gene Aliases: HFE2, HFE2A, HJV, JH, RGMC, hemochromatosis type 2(Juvenile), Haemojuvelin, RGM domain family member C

Pathways: Transition Metal Ion Homeostasis