TRKB Kit ELISA

N° du produit ABIN5657339

Détail du produit TRKB Kit ELISA

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2). No significant cross-reactivity or interference between Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Autre désignation: Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2 Produits)

Synonymes: trkb Kit ELISA, trkb-b Kit ELISA, xtrkb Kit ELISA, si:ch211-220b11.1 Kit ELISA, trkB2 Kit ELISA, NTRK2 Kit ELISA, ntrk2 Kit ELISA, gp145-trkb Kit ELISA, TRKB Kit ELISA, GP145-TrkB Kit ELISA, trk-B Kit ELISA, AI848316 Kit ELISA, C030027L06Rik Kit ELISA, Tkrb Kit ELISA, trkB Kit ELISA, RATTRKB1 Kit ELISA, TRKB1 Kit ELISA, Trk-B Kit ELISA, TrkB Kit ELISA, neurotrophic receptor tyrosine kinase 2 Kit ELISA, neurotrophic receptor tyrosine kinase 2 L homeolog Kit ELISA, neurotrophic tyrosine kinase, receptor, type 2b Kit ELISA, neurotrophic receptor tyrosine kinase 2 S homeolog Kit ELISA, neurotrophic tyrosine kinase, receptor, type 2 Kit ELISA, NTRK2 Kit ELISA, ntrk2.L Kit ELISA, ntrk2b Kit ELISA, ntrk2.S Kit ELISA, ntrk2 Kit ELISA, Ntrk2 Kit ELISA

Sujet:

Gene Name: Neurotrophic Tyrosine Kinase Receptor Type 2

Gene Aliases: GP145-TrkB, TRK-B, BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B, TrkB tyrosine kinase

ID gène: 4915

UniProt: Q16620

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport