KIT Kit ELISA
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- Antigène Voir toutes KIT Kits ELISA
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Human SCF Receptor ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes human SCF R.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes KIT Kits ELISA
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- Autre désignation
- SCF R (KIT Produits)
- Synonymes
- C-Kit Kit ELISA, CD117 Kit ELISA, PBT Kit ELISA, SCFR Kit ELISA, Bs Kit ELISA, Fdc Kit ELISA, Gsfsco1 Kit ELISA, Gsfsco5 Kit ELISA, Gsfsow3 Kit ELISA, SCO1 Kit ELISA, SCO5 Kit ELISA, SOW3 Kit ELISA, Ssm Kit ELISA, Tr-kit Kit ELISA, W Kit ELISA, c-KIT Kit ELISA, c-kit Kit ELISA, MGF Kit ELISA, KIT Kit ELISA, C-KIT Kit ELISA, kit Kit ELISA, receptor Kit ELISA, sparse Kit ELISA, Xkl-1 Kit ELISA, kit-a Kit ELISA, kit-b Kit ELISA, kl1-A Kit ELISA, krk1 Kit ELISA, pbt Kit ELISA, scfr Kit ELISA, KIT proto-oncogene receptor tyrosine kinase Kit ELISA, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Kit ELISA, KIT proto-oncogene receptor tyrosine kinase S homeolog Kit ELISA, KIT Kit ELISA, Kit Kit ELISA, kita Kit ELISA, kit Kit ELISA, kit.S Kit ELISA
- ID gène
- 3815
- UniProt
- P10721
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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