TNFRSF1A Kit ELISA (Tumor Necrosis Factor Receptor Superfamily, Member 1A)

Details for Product TNFRSF1A Kit ELISA No. ABIN625426
Antigène
  • CD120a
  • FPF
  • MS5
  • TBP1
  • TNF-R
  • TNF-R-I
  • TNF-R55
  • TNFAR
  • TNFR1
  • TNFR1-d2
  • TNFR55
  • TNFR60
  • p55
  • p55-R
  • p60
  • TNF-R1
  • TNF-alphaR1
  • TNFRI
  • TNFRp55
  • TNFalpha-R1
  • Tnfr-2
  • Tnfr1
  • fpf
  • tbp1
  • p55-r
  • tnf-r
  • tnfar
  • tnfr1
  • cd120a
  • tnfr55
  • tnfr60
  • xtnfr1
  • wu:fj65b10
  • zgc:85914
  • TNFR I
  • TNFRSF1A
  • TNFA
  • cTNF
  • TNF receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1a
  • TNF receptor superfamily member 1A S homeolog
  • tumor necrosis factor
  • TNFRSF1A
  • Tnfrsf1a
  • tnfrsf1a.S
  • tnfrsf1a
  • TNF
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
6
6
6
3
3
2
2
1
1
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
1-300 pg/mL
Seuil minimal de détection
1 pg/mL
Application
ELISA
Options
Fonction Mouse TNF RI (TNFRSF1A) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This ELISA antibody pair detects mouse sTNFRI. Other species not determined.
Sensibilité 1 pg/mL
Attributs du produit
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others TNFRSF1A products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation TNF RI
Sujet Gene Names: Tnfrsf1a Tnfr-1 Tnfr1
Protein names: Tumor necrosis factor receptor superfamily member 1A (Tumor necrosis factor receptor 1) (TNF-R1) (Tumor necrosis factor receptor type I) (TNF-RI) (TNFR-I) (p55) (p60) (CD antigen CD120a)
ID gène 21937
UniProt P25118
Pathways Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C
Indications d'application Recommended Dilution for serum and plasma samples5 - 50 fold
Volume d'échantillon 100 μL
Plaque Pre-coated
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Stock -20 °C
Stockage commentaire The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption 6 months
Images (Fournisseur)
Image no. 1 for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit (ABIN625426) Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit
Produit citée dans: Vergori, Lauret, Gaceb, Beauvillain, Andriantsitohaina, Martinez: "PPAR? regulates endothelial progenitor cell maturation and myeloid lineage differentiation through a NADPH oxidase-dependent mechanism in mice." dans: Stem cells (Dayton, Ohio), Vol. 33, Issue 4, pp. 1292-303, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Sample: Conditioned Media (BM dervied cells treated with M-CSF)

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