TRKB Kit ELISA

N° du produit ABIN6719696

Détail du produit TRKB Kit ELISA

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.8 pg/mL - 6000 pg/mL

Seuil minimal de détection: 93.8 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BDNF tropomyosine receptor kinase B, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, Ntrk2, NTRK2_HUMAN, Trk-B, TRKB, TrkB tyrosine kinase, Tyrosine kinase receptor B

Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32-H439.

Gene Name: NTRK2

Production: Natural and recombinant mouse TrkB. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: C32-H429

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.8pg/mL mouse trkB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates or tissue lysates to each empty well. We recommend that each mouse trkB standard solution and each sample is measured in duplicate.

ELISA Dilution: 93.8pg/mL-6000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Autre désignation: TrkB (NTRK2 Produits)

Synonymes: trkb Kit ELISA, trkb-b Kit ELISA, xtrkb Kit ELISA, si:ch211-220b11.1 Kit ELISA, trkB2 Kit ELISA, NTRK2 Kit ELISA, ntrk2 Kit ELISA, gp145-trkb Kit ELISA, TRKB Kit ELISA, GP145-TrkB Kit ELISA, trk-B Kit ELISA, AI848316 Kit ELISA, C030027L06Rik Kit ELISA, Tkrb Kit ELISA, trkB Kit ELISA, RATTRKB1 Kit ELISA, TRKB1 Kit ELISA, Trk-B Kit ELISA, TrkB Kit ELISA, neurotrophic receptor tyrosine kinase 2 Kit ELISA, neurotrophic receptor tyrosine kinase 2 L homeolog Kit ELISA, neurotrophic tyrosine kinase, receptor, type 2b Kit ELISA, neurotrophic receptor tyrosine kinase 2 S homeolog Kit ELISA, neurotrophic tyrosine kinase, receptor, type 2 Kit ELISA, NTRK2 Kit ELISA, ntrk2.L Kit ELISA, ntrk2b Kit ELISA, ntrk2.S Kit ELISA, ntrk2 Kit ELISA, Ntrk2 Kit ELISA

ID gène: 18212

NCBI Accession: NP_001020245

UniProt: P15209

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport