FCGR2B Kit ELISA

N° du produit ABIN6951585

Détail du produit FCGR2B Kit ELISA

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Rat Fc gamma RIIB/CD32b ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Autre désignation: Fc gamma RIIB (CD32b (FCGR2B Produits)

Synonymes: CD32 Kit ELISA, CD32B Kit ELISA, FCG2 Kit ELISA, FCGR2 Kit ELISA, IGFR2 Kit ELISA, FCGR2B Kit ELISA, AI528646 Kit ELISA, F630109E10Rik Kit ELISA, Fc[g]RII Kit ELISA, FcgRII Kit ELISA, Fcgr2 Kit ELISA, Fcgr2a Kit ELISA, Fcr-2 Kit ELISA, Fcr-3 Kit ELISA, Ly-17 Kit ELISA, Ly-m20 Kit ELISA, LyM-1 Kit ELISA, Lym-1 Kit ELISA, FCRII Kit ELISA, FcRII Kit ELISA, Fc fragment of IgG receptor IIb Kit ELISA, Fc fragment of IgG, low affinity IIb, receptor (CD32) Kit ELISA, Fc receptor, IgG, low affinity IIb Kit ELISA, low affinity immunoglobulin gamma Fc region receptor II Kit ELISA, FCGR2B Kit ELISA, Fcgr2b Kit ELISA, LOC100856270 Kit ELISA

ID gène: 289211

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling