TNFSF13 Kit ELISA

N° du produit ABIN6951655

Détail du produit TNFSF13 Kit ELISA

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Rat APRIL ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Autre désignation: APRIL (TNFSF13 Produits)

Synonymes: APRIL Kit ELISA, CD256 Kit ELISA, TALL-2 Kit ELISA, TALL2 Kit ELISA, TRDL-1 Kit ELISA, ZTNF2 Kit ELISA, 2310026N09Rik Kit ELISA, April Kit ELISA, Tall2 Kit ELISA, Trdl1 Kit ELISA, SLAN Kit ELISA, ANP32B Kit ELISA, april Kit ELISA, tnf13 Kit ELISA, TNLG7B Kit ELISA, TNF superfamily member 13 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 13 Kit ELISA, acidic leucine-rich nuclear phosphoprotein 32 family member B Kit ELISA, TNFSF13 Kit ELISA, Tnfsf13 Kit ELISA, LOC494442 Kit ELISA, tnfsf13 Kit ELISA

ID gène: 287437

UniProt: Q5PQL1

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response