GSK3 alpha Kit ELISA

N° du produit ABIN7830689

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain GSK3 alpha.

Aperçu rapide pour GSK3 alpha Kit ELISA (ABIN7830689)

Antigène: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit GSK3 alpha Kit ELISA

Fonction: GSK3A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GSK3A expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Autre désignation: GSK3A

Sujet:

Synonyms: DKFZp686D0638

Gene Description: glycogen synthase kinase 3 alpha

ID gène: 2931

UniProt: P49840

Pathways: Signalisation PI3K-Akt, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, L'effet Warburg